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qiaamp dna extraction kit  (Qiagen)


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    Qiagen qiaamp dna extraction kit
    Qiaamp Dna Extraction Kit, supplied by Qiagen, used in various techniques. Bioz Stars score: 99/100, based on 56155 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/dna+extraction+kit/QIAamp+DNA+Mini+Kit/pmc13676301-131-16-20
    Average 99 stars, based on 56155 article reviews
    qiaamp dna extraction kit - by Bioz Stars, 2026-10
    99/100 stars

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    Related Articles

    Incubation:

    Article Title: BRAF V595E detection, uroplakin II expression and PAS staining for diagnosing canine urothelial carcinoma
    Article Snippet: .. All three sample types were digested with proteinase K and incubated at 56 °C, following the protocol for the DNA extraction kit (Qiagen, DNeasy Blood & Tissue Kit). ..

    Article Title: Bacteriocin gene-mediated ecological adaptation of Bifidobacterium breve in the adult human gut
    Article Snippet: The isolates were anaerobically cultured on modified de Man, Rogosa, and Sharpe (mMRS) agar and broth media supplemented with 0.05% (w/v) L-cysteine, using a Whitley DG250 anaerobic workstation (Don Whitley Scientific Limited, Shipley, UK). .. Cultures were incubated at 37°C for 24 h. Genomic DNA was extracted from pure cultures using a commercial DNA extraction kit (Qiagen, cat.51604) according to the manufacturer’s instructions. ..

    DNA Extraction:

    Article Title: BRAF V595E detection, uroplakin II expression and PAS staining for diagnosing canine urothelial carcinoma
    Article Snippet: .. All three sample types were digested with proteinase K and incubated at 56 °C, following the protocol for the DNA extraction kit (Qiagen, DNeasy Blood & Tissue Kit). ..

    Article Title: Bacteriocin gene-mediated ecological adaptation of Bifidobacterium breve in the adult human gut
    Article Snippet: The isolates were anaerobically cultured on modified de Man, Rogosa, and Sharpe (mMRS) agar and broth media supplemented with 0.05% (w/v) L-cysteine, using a Whitley DG250 anaerobic workstation (Don Whitley Scientific Limited, Shipley, UK). .. Cultures were incubated at 37°C for 24 h. Genomic DNA was extracted from pure cultures using a commercial DNA extraction kit (Qiagen, cat.51604) according to the manufacturer’s instructions. ..

    Article Title: Phenotypic and Genotypic Analyses of Antimicrobial Resistance Patterns of Staphylococcus aureus Isolates From Outpatient Blood Samples in Mukuru Slum, Nairobi, Kenya
    Article Snippet: S. aureus ATCC 25923 was used for quality control, while distilled water (DH 2 O) was used as a negative control [ ]. .. Genomic DNA was extracted from overnight bacterial cultures using a commercial DNA extraction kit (QIAamp DNA Mini Kit, Qiagen, Hilden, Germany) following the manufacturer’s instructions. ..

    Article Title: Melting temperature mapping for rapid pathogen identification in neonatal bloodstream infections: A prospective study.
    Article Snippet: Bloodstream infections remain a lifethreatening condition in infants admitted to the neonatal intensive care unit (NICU), particularly premature infants.. Despite improvements in diagnostic techniques and the availability of various broadspectrum antibiotics, neonatal sepsis remains a leading cause of neonatal morbidity and mortality worldwide.. The symptoms of neonates with sepsis are nonspecific and diverse, making early diagnosis sometimes tricky.

    Article Title: Establishment and in vitro validation of a sheep-specific, low-cost TLR7/8 agonist R848-mediated X/Y sperm separation system
    Article Snippet: .. All reagents and antibodies were sourced with complete manufacturer information: R848 (Cat. No. S8120), ATP detection kit (Cat. No. BC0300), glutathione (GSH) detection kit (Cat. No. BC1170), JC-1 fluorescent probe (Cat. No. CA2060), SYBR14/PI double staining kit (Cat. No. CA1320), FITC-PNA (Cat. No. C7622), DAPI (Cat. No. C1002) were purchased from Solarbio Science & Technology Co., Ltd. (Beijing, China); Fetal Bovine Serum (FBS, Cat. No. 10099141), DMEM medium (Cat. No. 11965092), TCM-199 medium (Cat. No. 31100035) were from Gibco (Thermo Fisher Scientific, Waltham, MA, USA); Protease inhibitor PMSF (Cat. No. 10837091001), phosphatase inhibitor cocktail (Cat. No. 04906845001) were from Roche Diagnostics (Basel, Switzerland); DNA extraction kit (QIAGEN DNeasy Blood & Tissue Kit, Cat. No. 69504), agarose (Lonza, Cat. No. 50004) were from QIAGEN (Hilden, Germany) and Lonza (Basel, Switzerland), respectively; Anti-TLR8 antibody (Abcam, Cat. No. ab134932, clone D13B12), anti-SRY antibody (Santa Cruz Biotechnology, Cat. No. sc-166466, clone C-19), HRP-labeled goat anti-rabbit IgG (Jackson ImmunoResearch, Cat. No. 111-035-003) were from Abcam (Cambridge, UK), Santa Cruz Biotechnology (Dallas, TX, USA), and Jackson ImmunoResearch (West Grove, PA, USA), respectively; Semen sorting solution and diluent I were self-prepared (detailed compositions below); Other reagents (heparin, caffeine, hyaluronidase, SOF medium) were of analytical grade (Sigma-Aldrich, St. Louis, MO, USA). ..

    Article Title: PEGylated Nano Lipopolyplexes with Synergistic Lipid Combinations Demonstrate High-Efficiency Nonviral Cellular Delivery of CRISPR/Cas9 Plasmids
    Article Snippet: .. Genomic DNA was extracted from both untreated and treated cells 60 h after nanoparticle treatment using a DNA extraction kit (Qiagen, #56304) according to the manufacturer’s instructions. .. DNA concentration and purity were assessed by using a NanoDrop spectrophotometer (Thermo Fisher Scientific).

    Article Title: The PPTC7/BNIP3/NIX axis induces cGAS/STING-mediated senescence and augments CAR-T efficacy by repressing tumor-intrinsic mitophagy.
    Article Snippet: Cytoplasmic and nuclear fractions were separated using a Cytoplasmic and Nuclear RNA Purification Kit (NGB-21000, Norgen Biotek, Canada). .. DNA from the cytoplasmic fraction was isolated with a DNA Extraction Kit (69506, Qiagen, Germany), and DNA concentration and purity were determined before downstream analysis. ..

    Article Title: A novel approach to HPV16 VLP construction and production with improved accessibility and particle sorting potential
    Article Snippet: .. Potential DNA content in the VLPs was analyzed using a DNA extraction kit (Qiagen PCR cleanup kit) and a disassembly buffer containing 10 mM DTT. ..

    Isolation:

    Article Title: Melting temperature mapping for rapid pathogen identification in neonatal bloodstream infections: A prospective study.
    Article Snippet: Bloodstream infections remain a lifethreatening condition in infants admitted to the neonatal intensive care unit (NICU), particularly premature infants.. Despite improvements in diagnostic techniques and the availability of various broadspectrum antibiotics, neonatal sepsis remains a leading cause of neonatal morbidity and mortality worldwide.. The symptoms of neonates with sepsis are nonspecific and diverse, making early diagnosis sometimes tricky.

    Article Title: The PPTC7/BNIP3/NIX axis induces cGAS/STING-mediated senescence and augments CAR-T efficacy by repressing tumor-intrinsic mitophagy.
    Article Snippet: Cytoplasmic and nuclear fractions were separated using a Cytoplasmic and Nuclear RNA Purification Kit (NGB-21000, Norgen Biotek, Canada). .. DNA from the cytoplasmic fraction was isolated with a DNA Extraction Kit (69506, Qiagen, Germany), and DNA concentration and purity were determined before downstream analysis. ..

    Double Staining:

    Article Title: Establishment and in vitro validation of a sheep-specific, low-cost TLR7/8 agonist R848-mediated X/Y sperm separation system
    Article Snippet: .. All reagents and antibodies were sourced with complete manufacturer information: R848 (Cat. No. S8120), ATP detection kit (Cat. No. BC0300), glutathione (GSH) detection kit (Cat. No. BC1170), JC-1 fluorescent probe (Cat. No. CA2060), SYBR14/PI double staining kit (Cat. No. CA1320), FITC-PNA (Cat. No. C7622), DAPI (Cat. No. C1002) were purchased from Solarbio Science & Technology Co., Ltd. (Beijing, China); Fetal Bovine Serum (FBS, Cat. No. 10099141), DMEM medium (Cat. No. 11965092), TCM-199 medium (Cat. No. 31100035) were from Gibco (Thermo Fisher Scientific, Waltham, MA, USA); Protease inhibitor PMSF (Cat. No. 10837091001), phosphatase inhibitor cocktail (Cat. No. 04906845001) were from Roche Diagnostics (Basel, Switzerland); DNA extraction kit (QIAGEN DNeasy Blood & Tissue Kit, Cat. No. 69504), agarose (Lonza, Cat. No. 50004) were from QIAGEN (Hilden, Germany) and Lonza (Basel, Switzerland), respectively; Anti-TLR8 antibody (Abcam, Cat. No. ab134932, clone D13B12), anti-SRY antibody (Santa Cruz Biotechnology, Cat. No. sc-166466, clone C-19), HRP-labeled goat anti-rabbit IgG (Jackson ImmunoResearch, Cat. No. 111-035-003) were from Abcam (Cambridge, UK), Santa Cruz Biotechnology (Dallas, TX, USA), and Jackson ImmunoResearch (West Grove, PA, USA), respectively; Semen sorting solution and diluent I were self-prepared (detailed compositions below); Other reagents (heparin, caffeine, hyaluronidase, SOF medium) were of analytical grade (Sigma-Aldrich, St. Louis, MO, USA). ..

    Protease Inhibitor:

    Article Title: Establishment and in vitro validation of a sheep-specific, low-cost TLR7/8 agonist R848-mediated X/Y sperm separation system
    Article Snippet: .. All reagents and antibodies were sourced with complete manufacturer information: R848 (Cat. No. S8120), ATP detection kit (Cat. No. BC0300), glutathione (GSH) detection kit (Cat. No. BC1170), JC-1 fluorescent probe (Cat. No. CA2060), SYBR14/PI double staining kit (Cat. No. CA1320), FITC-PNA (Cat. No. C7622), DAPI (Cat. No. C1002) were purchased from Solarbio Science & Technology Co., Ltd. (Beijing, China); Fetal Bovine Serum (FBS, Cat. No. 10099141), DMEM medium (Cat. No. 11965092), TCM-199 medium (Cat. No. 31100035) were from Gibco (Thermo Fisher Scientific, Waltham, MA, USA); Protease inhibitor PMSF (Cat. No. 10837091001), phosphatase inhibitor cocktail (Cat. No. 04906845001) were from Roche Diagnostics (Basel, Switzerland); DNA extraction kit (QIAGEN DNeasy Blood & Tissue Kit, Cat. No. 69504), agarose (Lonza, Cat. No. 50004) were from QIAGEN (Hilden, Germany) and Lonza (Basel, Switzerland), respectively; Anti-TLR8 antibody (Abcam, Cat. No. ab134932, clone D13B12), anti-SRY antibody (Santa Cruz Biotechnology, Cat. No. sc-166466, clone C-19), HRP-labeled goat anti-rabbit IgG (Jackson ImmunoResearch, Cat. No. 111-035-003) were from Abcam (Cambridge, UK), Santa Cruz Biotechnology (Dallas, TX, USA), and Jackson ImmunoResearch (West Grove, PA, USA), respectively; Semen sorting solution and diluent I were self-prepared (detailed compositions below); Other reagents (heparin, caffeine, hyaluronidase, SOF medium) were of analytical grade (Sigma-Aldrich, St. Louis, MO, USA). ..

    Tissue:

    Article Title: Establishment and in vitro validation of a sheep-specific, low-cost TLR7/8 agonist R848-mediated X/Y sperm separation system
    Article Snippet: .. All reagents and antibodies were sourced with complete manufacturer information: R848 (Cat. No. S8120), ATP detection kit (Cat. No. BC0300), glutathione (GSH) detection kit (Cat. No. BC1170), JC-1 fluorescent probe (Cat. No. CA2060), SYBR14/PI double staining kit (Cat. No. CA1320), FITC-PNA (Cat. No. C7622), DAPI (Cat. No. C1002) were purchased from Solarbio Science & Technology Co., Ltd. (Beijing, China); Fetal Bovine Serum (FBS, Cat. No. 10099141), DMEM medium (Cat. No. 11965092), TCM-199 medium (Cat. No. 31100035) were from Gibco (Thermo Fisher Scientific, Waltham, MA, USA); Protease inhibitor PMSF (Cat. No. 10837091001), phosphatase inhibitor cocktail (Cat. No. 04906845001) were from Roche Diagnostics (Basel, Switzerland); DNA extraction kit (QIAGEN DNeasy Blood & Tissue Kit, Cat. No. 69504), agarose (Lonza, Cat. No. 50004) were from QIAGEN (Hilden, Germany) and Lonza (Basel, Switzerland), respectively; Anti-TLR8 antibody (Abcam, Cat. No. ab134932, clone D13B12), anti-SRY antibody (Santa Cruz Biotechnology, Cat. No. sc-166466, clone C-19), HRP-labeled goat anti-rabbit IgG (Jackson ImmunoResearch, Cat. No. 111-035-003) were from Abcam (Cambridge, UK), Santa Cruz Biotechnology (Dallas, TX, USA), and Jackson ImmunoResearch (West Grove, PA, USA), respectively; Semen sorting solution and diluent I were self-prepared (detailed compositions below); Other reagents (heparin, caffeine, hyaluronidase, SOF medium) were of analytical grade (Sigma-Aldrich, St. Louis, MO, USA). ..

    Concentration Assay:

    Article Title: The PPTC7/BNIP3/NIX axis induces cGAS/STING-mediated senescence and augments CAR-T efficacy by repressing tumor-intrinsic mitophagy.
    Article Snippet: Cytoplasmic and nuclear fractions were separated using a Cytoplasmic and Nuclear RNA Purification Kit (NGB-21000, Norgen Biotek, Canada). .. DNA from the cytoplasmic fraction was isolated with a DNA Extraction Kit (69506, Qiagen, Germany), and DNA concentration and purity were determined before downstream analysis. ..

    Polymerase Chain Reaction:

    Article Title: A novel approach to HPV16 VLP construction and production with improved accessibility and particle sorting potential
    Article Snippet: .. Potential DNA content in the VLPs was analyzed using a DNA extraction kit (Qiagen PCR cleanup kit) and a disassembly buffer containing 10 mM DTT. ..



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    Optimization of RPA primers and CRISPR/Cas12a detection for the Mycoplasma bovis uvrC gene. (A) Schematic of p‐uvrC generation by restriction cloning of a 558‐bp uvrC fragment, which was used as the standard DNA template. (B) Screening of eight RPA primer pairs (PP1–PP8) targeting uvrC; PP3–PP8 produced strong, specific amplicons, and target amplification was also detected in DNA from M. bovis ‐positive bison lung. (C) Sequence of three CRISPR guide RNAs (gRNA1–gRNA3) designed within the amplified uvrC region adjacent to 5′‐TTTG‐3′ PAM sites. (D) Comparison of Cas12a fluorescence kinetics showed that all three guides detected uvrC, with gRNA3 producing the fastest and strongest signal. (E) Optimization of ssDNA‐FQ reporter concentration identified 0.75 μM as the best condition for visual detection with minimal background, although maximal fluorescence was reached at 5 μM. (F) All guide RNA combinations produced detectable fluorescence with similar kinetics, although gRNA2 + 3 and gRNA1 + 2 + 3 showed slightly faster onset.

    Journal: MicrobiologyOpen

    Article Title: Synergistic Integration of Recombinase Polymerase Amplification and CRISPR–Cas12a Enables High‐Sensitivity Detection of Mycoplasma bovis

    doi: 10.1002/mbo3.70435

    Figure Lengend Snippet: Optimization of RPA primers and CRISPR/Cas12a detection for the Mycoplasma bovis uvrC gene. (A) Schematic of p‐uvrC generation by restriction cloning of a 558‐bp uvrC fragment, which was used as the standard DNA template. (B) Screening of eight RPA primer pairs (PP1–PP8) targeting uvrC; PP3–PP8 produced strong, specific amplicons, and target amplification was also detected in DNA from M. bovis ‐positive bison lung. (C) Sequence of three CRISPR guide RNAs (gRNA1–gRNA3) designed within the amplified uvrC region adjacent to 5′‐TTTG‐3′ PAM sites. (D) Comparison of Cas12a fluorescence kinetics showed that all three guides detected uvrC, with gRNA3 producing the fastest and strongest signal. (E) Optimization of ssDNA‐FQ reporter concentration identified 0.75 μM as the best condition for visual detection with minimal background, although maximal fluorescence was reached at 5 μM. (F) All guide RNA combinations produced detectable fluorescence with similar kinetics, although gRNA2 + 3 and gRNA1 + 2 + 3 showed slightly faster onset.

    Article Snippet: The target band was excised and purified using the Monarch DNA Gel Extraction Kit (New England Biolabs).

    Techniques: CRISPR, Cloning, Screening, Produced, Amplification, Sequencing, Comparison, Fluorescence, Concentration Assay

    Journal: iScience

    Article Title: Tregs transduced with a Ro60-reactive TCR have increased immunosuppressive functions in Sjögren’s disease

    doi: 10.1016/j.isci.2026.117642

    Figure Lengend Snippet:

    Article Snippet: DNA Plasmid Extraction Kit , Qiagen , Cat No. 27104.

    Techniques: Isolation, Cell Isolation, DNA Extraction, Quantitation Assay, Plasmid Preparation, Extraction, Recombinant, Expressing, Software

    Journal: iScience

    Article Title: Tregs transduced with a Ro60-reactive TCR have increased immunosuppressive functions in Sjögren’s disease

    doi: 10.1016/j.isci.2026.117642

    Figure Lengend Snippet:

    Article Snippet: Genomic DNA Extraction Kit , New England Biolabs , Cat No. T3010L.

    Techniques: Isolation, Cell Isolation, DNA Extraction, Quantitation Assay, Plasmid Preparation, Extraction, Recombinant, Expressing, Software

    Signatures of genes suspected to be horizontally transferred from bacteria to leafhoppers. (A) Genome and transcriptome coverage of RdCDC9 ( Id09G000860 ). The read depths of genome resequencing and RNA sequencing data are log‐transformed and plotted along the focal genomic region, with numbers indicating the range of depths. Splice junctions in RdCDC9 inferred from the RNA sequencing reads are indicated by red lines. The two fragments used for PCR cloning of genomic DNA are represented as red segments. (B) Gene structures of the CDC genes in three leafhopper species. Green and pink rectangles represent the CDSs and UTRs, respectively, and the black lines represent introns. (C) Genome fragments cloned from Ne. cincticeps , Ne. nigropictus , and R. dorsalis genomic DNA. For the intron‐bearing genes RdCDC6 and RdCDC9 , two pairs of primers targeting the intron regions were used. Bar plots represent the expression levels of all CDCs detected by RT‐qPCR. (D) Identification of the RdCDC protein by immunoprecipitation‐mass spectrometry (IP‐MS). The R. dorsalis adult lysate was immunoprecipitated using IgG or an RdCDC9 antibody and analyzed by mass spectrometry to identify candidate proteins. (E) Hierarchical clustering results of CDC family members based on protein similarity. All genes from leafhoppers are clustered together and grouped with the CDCs from Firmicutes and Actinobacteria, suggesting that they may have originated from an ancient horizontal gene transfer event. The TCDB accession number and the common name or species of origin are provided for each protein used to construct the phylogeny. Predictions of the protein domains revealed that all leafhopper CDCs contained a region belonging to the thiol‐activated cytolysin superfamily (IPR036359). (F) Heatmap showing the average relative synonymous codon usage (RSCU) of leafhopper CDCs , bacterial CDCs ( PFO , LLO , and SLO ), and conserved single‐copy genes of the three leafhopper species. The gradient from dark blue to dark red indicates increasing RSCU values from low to high. LLO, listeriolysin O; PFO, perfringolysin O; SLO, streptolysin O.

    Journal: iMeta

    Article Title: Domesticated bacterial origin genes regulate homeostasis of the insect gut microbiome and empower adaptation

    doi: 10.1002/imt2.70179

    Figure Lengend Snippet: Signatures of genes suspected to be horizontally transferred from bacteria to leafhoppers. (A) Genome and transcriptome coverage of RdCDC9 ( Id09G000860 ). The read depths of genome resequencing and RNA sequencing data are log‐transformed and plotted along the focal genomic region, with numbers indicating the range of depths. Splice junctions in RdCDC9 inferred from the RNA sequencing reads are indicated by red lines. The two fragments used for PCR cloning of genomic DNA are represented as red segments. (B) Gene structures of the CDC genes in three leafhopper species. Green and pink rectangles represent the CDSs and UTRs, respectively, and the black lines represent introns. (C) Genome fragments cloned from Ne. cincticeps , Ne. nigropictus , and R. dorsalis genomic DNA. For the intron‐bearing genes RdCDC6 and RdCDC9 , two pairs of primers targeting the intron regions were used. Bar plots represent the expression levels of all CDCs detected by RT‐qPCR. (D) Identification of the RdCDC protein by immunoprecipitation‐mass spectrometry (IP‐MS). The R. dorsalis adult lysate was immunoprecipitated using IgG or an RdCDC9 antibody and analyzed by mass spectrometry to identify candidate proteins. (E) Hierarchical clustering results of CDC family members based on protein similarity. All genes from leafhoppers are clustered together and grouped with the CDCs from Firmicutes and Actinobacteria, suggesting that they may have originated from an ancient horizontal gene transfer event. The TCDB accession number and the common name or species of origin are provided for each protein used to construct the phylogeny. Predictions of the protein domains revealed that all leafhopper CDCs contained a region belonging to the thiol‐activated cytolysin superfamily (IPR036359). (F) Heatmap showing the average relative synonymous codon usage (RSCU) of leafhopper CDCs , bacterial CDCs ( PFO , LLO , and SLO ), and conserved single‐copy genes of the three leafhopper species. The gradient from dark blue to dark red indicates increasing RSCU values from low to high. LLO, listeriolysin O; PFO, perfringolysin O; SLO, streptolysin O.

    Article Snippet: DNA was extracted using an Animal Genomic DNA Quick Extraction Kit for PCR Analysis (Beyotime, D0065S).

    Techniques: Bacteria, RNA Sequencing, Transformation Assay, PCR Cloning, Clone Assay, Expressing, Quantitative RT-PCR, Immunoprecipitation, Mass Spectrometry, Protein-Protein interactions, Construct